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	<title>Comments on: Government surplus DNA Sequencer, $200 to a good&#160;home</title>
	<atom:link href="http://boingboing.net/2013/01/17/government-surplus-dna-sequenc.html/feed" rel="self" type="application/rss+xml" />
	<link>http://boingboing.net/2013/01/17/government-surplus-dna-sequenc.html</link>
	<description>Brain candy for Happy Mutants</description>
	<lastBuildDate>Sat, 25 May 2013 21:13:00 +0000</lastBuildDate>
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		<title>By: Chris Slowik</title>
		<link>http://boingboing.net/2013/01/17/government-surplus-dna-sequenc.html#comment-1633132</link>
		<dc:creator>Chris Slowik</dc:creator>
		<pubDate>Mon, 21 Jan 2013 17:41:00 +0000</pubDate>
		<guid isPermaLink="false">http://boingboing.net/?p=206335#comment-1633132</guid>
		<description>the price is probably more because its a piece of science history, not because its feasible to use today =]</description>
		<content:encoded><![CDATA[<p>the price is probably more because its a piece of science history, not because its feasible to use today =]</p>
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		<title>By: Roger Mercer</title>
		<link>http://boingboing.net/2013/01/17/government-surplus-dna-sequenc.html#comment-1631354</link>
		<dc:creator>Roger Mercer</dc:creator>
		<pubDate>Fri, 18 Jan 2013 18:58:00 +0000</pubDate>
		<guid isPermaLink="false">http://boingboing.net/?p=206335#comment-1631354</guid>
		<description>We have a HiSeq 2500 (the current top of the line in next-gen sequencing) in my lab. I swear, when it get&#039;s surplussed in five years because we have disposable-chip single-molecule realtime sequencers, I&#039;m going to buy the sucker and turn it into the world&#039;s most awesome kegerator.</description>
		<content:encoded><![CDATA[<p>We have a HiSeq 2500 (the current top of the line in next-gen sequencing) in my lab. I swear, when it get&#8217;s surplussed in five years because we have disposable-chip single-molecule realtime sequencers, I&#8217;m going to buy the sucker and turn it into the world&#8217;s most awesome kegerator.</p>
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		<title>By: Preston Sturges</title>
		<link>http://boingboing.net/2013/01/17/government-surplus-dna-sequenc.html#comment-1630951</link>
		<dc:creator>Preston Sturges</dc:creator>
		<pubDate>Fri, 18 Jan 2013 08:10:00 +0000</pubDate>
		<guid isPermaLink="false">http://boingboing.net/?p=206335#comment-1630951</guid>
		<description>There&#039;s a DC power supply, a thermoelectric cooling unit, a tiny recirculating pump, not much else......</description>
		<content:encoded><![CDATA[<p>There&#8217;s a DC power supply, a thermoelectric cooling unit, a tiny recirculating pump, not much else&#8230;&#8230;</p>
]]></content:encoded>
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	<item>
		<title>By: yadayada</title>
		<link>http://boingboing.net/2013/01/17/government-surplus-dna-sequenc.html#comment-1630907</link>
		<dc:creator>yadayada</dc:creator>
		<pubDate>Fri, 18 Jan 2013 06:31:00 +0000</pubDate>
		<guid isPermaLink="false">http://boingboing.net/?p=206335#comment-1630907</guid>
		<description>Thanks for the tip.

Sincerely,
Dr. Moreau </description>
		<content:encoded><![CDATA[<p>Thanks for the tip.</p>
<p>Sincerely,<br />
Dr. Moreau </p>
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		<title>By: Daemonworks</title>
		<link>http://boingboing.net/2013/01/17/government-surplus-dna-sequenc.html#comment-1630888</link>
		<dc:creator>Daemonworks</dc:creator>
		<pubDate>Fri, 18 Jan 2013 06:01:00 +0000</pubDate>
		<guid isPermaLink="false">http://boingboing.net/?p=206335#comment-1630888</guid>
		<description>We need more Moreau, not less. </description>
		<content:encoded><![CDATA[<p>We need more Moreau, not less. </p>
]]></content:encoded>
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	<item>
		<title>By: Ed Ligget. Tuba.</title>
		<link>http://boingboing.net/2013/01/17/government-surplus-dna-sequenc.html#comment-1630859</link>
		<dc:creator>Ed Ligget. Tuba.</dc:creator>
		<pubDate>Fri, 18 Jan 2013 04:30:00 +0000</pubDate>
		<guid isPermaLink="false">http://boingboing.net/?p=206335#comment-1630859</guid>
		<description>I think you guys who are yelling &quot;obsolete!&quot; are forgetting what a wealth of take-out parts this would contain.</description>
		<content:encoded><![CDATA[<p>I think you guys who are yelling &#8220;obsolete!&#8221; are forgetting what a wealth of take-out parts this would contain.</p>
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		<title>By: nixiebunny</title>
		<link>http://boingboing.net/2013/01/17/government-surplus-dna-sequenc.html#comment-1630843</link>
		<dc:creator>nixiebunny</dc:creator>
		<pubDate>Fri, 18 Jan 2013 04:00:00 +0000</pubDate>
		<guid isPermaLink="false">http://boingboing.net/?p=206335#comment-1630843</guid>
		<description>A baseball bat, more like.

PC LOAD LETTER
</description>
		<content:encoded><![CDATA[<p>A baseball bat, more like.</p>
<p>PC LOAD LETTER</p>
]]></content:encoded>
	</item>
	<item>
		<title>By: Preston Sturges</title>
		<link>http://boingboing.net/2013/01/17/government-surplus-dna-sequenc.html#comment-1630841</link>
		<dc:creator>Preston Sturges</dc:creator>
		<pubDate>Fri, 18 Jan 2013 03:54:00 +0000</pubDate>
		<guid isPermaLink="false">http://boingboing.net/?p=206335#comment-1630841</guid>
		<description>You could probably get bitter ex gradstudents to pay a dollar a pop to shoot it with a revolver. </description>
		<content:encoded><![CDATA[<p>You could probably get bitter ex gradstudents to pay a dollar a pop to shoot it with a revolver. </p>
]]></content:encoded>
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		<title>By: nixiebunny</title>
		<link>http://boingboing.net/2013/01/17/government-surplus-dna-sequenc.html#comment-1630826</link>
		<dc:creator>nixiebunny</dc:creator>
		<pubDate>Fri, 18 Jan 2013 03:23:00 +0000</pubDate>
		<guid isPermaLink="false">http://boingboing.net/?p=206335#comment-1630826</guid>
		<description>This thing would be a lot of fun to disassemble.  

But not for $200 plus shipping. </description>
		<content:encoded><![CDATA[<p>This thing would be a lot of fun to disassemble.  </p>
<p>But not for $200 plus shipping. </p>
]]></content:encoded>
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		<title>By: Warren_Terra</title>
		<link>http://boingboing.net/2013/01/17/government-surplus-dna-sequenc.html#comment-1630795</link>
		<dc:creator>Warren_Terra</dc:creator>
		<pubDate>Fri, 18 Jan 2013 02:38:00 +0000</pubDate>
		<guid isPermaLink="false">http://boingboing.net/?p=206335#comment-1630795</guid>
		<description>Well, if you can remove it and control it properly, it probably contains quite a good electrophoresis power supply, and those aren&#039;t cheap - though you&#039;d need to be able to set the voltage, amperage, and timing, and those are probably pre-programmed, and there&#039;s probably no display. Also, it presumably has a bunch of electronically controlled liquid-handling components.</description>
		<content:encoded><![CDATA[<p>Well, if you can remove it and control it properly, it probably contains quite a good electrophoresis power supply, and those aren&#8217;t cheap &#8211; though you&#8217;d need to be able to set the voltage, amperage, and timing, and those are probably pre-programmed, and there&#8217;s probably no display. Also, it presumably has a bunch of electronically controlled liquid-handling components.</p>
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		<title>By: robuluz</title>
		<link>http://boingboing.net/2013/01/17/government-surplus-dna-sequenc.html#comment-1630796</link>
		<dc:creator>robuluz</dc:creator>
		<pubDate>Fri, 18 Jan 2013 02:38:00 +0000</pubDate>
		<guid isPermaLink="false">http://boingboing.net/?p=206335#comment-1630796</guid>
		<description>Well, that&#039;s just, like, your opinion, man.</description>
		<content:encoded><![CDATA[<p>Well, that&#8217;s just, like, your opinion, man.</p>
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		<title>By: voiceinthedistance</title>
		<link>http://boingboing.net/2013/01/17/government-surplus-dna-sequenc.html#comment-1630791</link>
		<dc:creator>voiceinthedistance</dc:creator>
		<pubDate>Fri, 18 Jan 2013 02:36:00 +0000</pubDate>
		<guid isPermaLink="false">http://boingboing.net/?p=206335#comment-1630791</guid>
		<description>It looks to me like it would transform into a pretty bad ass dorm fridge/photocopy machine.  With a few additional parts, of course.  </description>
		<content:encoded><![CDATA[<p>It looks to me like it would transform into a pretty bad ass dorm fridge/photocopy machine.  With a few additional parts, of course.  </p>
]]></content:encoded>
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		<title>By: Donald Petersen</title>
		<link>http://boingboing.net/2013/01/17/government-surplus-dna-sequenc.html#comment-1630779</link>
		<dc:creator>Donald Petersen</dc:creator>
		<pubDate>Fri, 18 Jan 2013 02:19:00 +0000</pubDate>
		<guid isPermaLink="false">http://boingboing.net/?p=206335#comment-1630779</guid>
		<description>Looks like it could be a fairly sturdy sawhorse.</description>
		<content:encoded><![CDATA[<p>Looks like it could be a fairly sturdy sawhorse.</p>
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		<title>By: CCinBmore</title>
		<link>http://boingboing.net/2013/01/17/government-surplus-dna-sequenc.html#comment-1630767</link>
		<dc:creator>CCinBmore</dc:creator>
		<pubDate>Fri, 18 Jan 2013 01:36:00 +0000</pubDate>
		<guid isPermaLink="false">http://boingboing.net/?p=206335#comment-1630767</guid>
		<description>Yeah, blown away as usual by the depth of knowledge here. But...

What ELSE can it be used for?
</description>
		<content:encoded><![CDATA[<p>Yeah, blown away as usual by the depth of knowledge here. But&#8230;</p>
<p>What ELSE can it be used for?</p>
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		<title>By: Jonathan Badger</title>
		<link>http://boingboing.net/2013/01/17/government-surplus-dna-sequenc.html#comment-1630764</link>
		<dc:creator>Jonathan Badger</dc:creator>
		<pubDate>Fri, 18 Jan 2013 01:22:00 +0000</pubDate>
		<guid isPermaLink="false">http://boingboing.net/?p=206335#comment-1630764</guid>
		<description>Yes, as others have mentioned this is very analogous to an obsolete ink-jet printer. This is true of nearly any piece of scientific equipment needing consumables and not just sequencers. Laboratories often have to upgrade equipment simply because the manufacturer has ceased selling the needed reagents. It&#039;s rarely a good deal to buy an old piece of scientific equipment unless you really know what you can do with it -- it&#039;s not like an old server which you probably could find some use for. </description>
		<content:encoded><![CDATA[<p>Yes, as others have mentioned this is very analogous to an obsolete ink-jet printer. This is true of nearly any piece of scientific equipment needing consumables and not just sequencers. Laboratories often have to upgrade equipment simply because the manufacturer has ceased selling the needed reagents. It&#8217;s rarely a good deal to buy an old piece of scientific equipment unless you really know what you can do with it &#8212; it&#8217;s not like an old server which you probably could find some use for. </p>
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		<title>By: edgore</title>
		<link>http://boingboing.net/2013/01/17/government-surplus-dna-sequenc.html#comment-1630719</link>
		<dc:creator>edgore</dc:creator>
		<pubDate>Thu, 17 Jan 2013 23:57:00 +0000</pubDate>
		<guid isPermaLink="false">http://boingboing.net/?p=206335#comment-1630719</guid>
		<description>Hmmmm...will this work off of the thermal-electric system in my volcano base?</description>
		<content:encoded><![CDATA[<p>Hmmmm&#8230;will this work off of the thermal-electric system in my volcano base?</p>
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		<title>By: Kylini</title>
		<link>http://boingboing.net/2013/01/17/government-surplus-dna-sequenc.html#comment-1630718</link>
		<dc:creator>Kylini</dc:creator>
		<pubDate>Thu, 17 Jan 2013 23:56:00 +0000</pubDate>
		<guid isPermaLink="false">http://boingboing.net/?p=206335#comment-1630718</guid>
		<description>Even if this were a laser-based Sanger sequencer (an ABI 3730, for example), the cost of running it would still be obscene. Spare parts aren&#039;t cheap and the reagent necessary to dye each base must always be purchased fresh at full cost. Think of ABI&#039;s &quot;Big Dye&quot; as printer ink.</description>
		<content:encoded><![CDATA[<p>Even if this were a laser-based Sanger sequencer (an ABI 3730, for example), the cost of running it would still be obscene. Spare parts aren&#8217;t cheap and the reagent necessary to dye each base must always be purchased fresh at full cost. Think of ABI&#8217;s &#8220;Big Dye&#8221; as printer ink.</p>
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		<title>By: cegev</title>
		<link>http://boingboing.net/2013/01/17/government-surplus-dna-sequenc.html#comment-1630671</link>
		<dc:creator>cegev</dc:creator>
		<pubDate>Thu, 17 Jan 2013 23:19:00 +0000</pubDate>
		<guid isPermaLink="false">http://boingboing.net/?p=206335#comment-1630671</guid>
		<description>I&#039;m essentially in agreement with Warren_Terra here: this is incredibly cheap because it&#039;s old enough that using it would be pointless. Actually, I&#039;d say the $200 price for this is rather high; the government should be happy to be able to give it away rather than pay for its disposal.

Even as far back as 2003, we couldn&#039;t find buyers for ABI 377s that were much better and easier to use than this sequencer; we had around 15 of them and I don&#039;t we would have been able to get more than $1,000 each or so. With ten years of progress after that, I&#039;d expect 377s and the like are less than $200, especially since major sequencing projects in the late 90s and early 00s used huge numbers of them. 

It&#039;s worth noting that some processes, like oligonucleotide synthesis and sequencing, are largely commoditized now if you&#039;re not doing something incredibly unusual. It would almost certainly be much more expensive, in terms of both materials and time, to use an old sequencer instead of just outsourcing the sequencing to a sequencing company.</description>
		<content:encoded><![CDATA[<p>I&#8217;m essentially in agreement with Warren_Terra here: this is incredibly cheap because it&#8217;s old enough that using it would be pointless. Actually, I&#8217;d say the $200 price for this is rather high; the government should be happy to be able to give it away rather than pay for its disposal.</p>
<p>Even as far back as 2003, we couldn&#8217;t find buyers for ABI 377s that were much better and easier to use than this sequencer; we had around 15 of them and I don&#8217;t we would have been able to get more than $1,000 each or so. With ten years of progress after that, I&#8217;d expect 377s and the like are less than $200, especially since major sequencing projects in the late 90s and early 00s used huge numbers of them. </p>
<p>It&#8217;s worth noting that some processes, like oligonucleotide synthesis and sequencing, are largely commoditized now if you&#8217;re not doing something incredibly unusual. It would almost certainly be much more expensive, in terms of both materials and time, to use an old sequencer instead of just outsourcing the sequencing to a sequencing company.</p>
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		<title>By: Warren_Terra</title>
		<link>http://boingboing.net/2013/01/17/government-surplus-dna-sequenc.html#comment-1630651</link>
		<dc:creator>Warren_Terra</dc:creator>
		<pubDate>Thu, 17 Jan 2013 23:02:00 +0000</pubDate>
		<guid isPermaLink="false">http://boingboing.net/?p=206335#comment-1630651</guid>
		<description>It&#039;s a gel electrophoresis system for using radioactively labeled nucleotides, after which the gel can be dried and exposed to film or to phosphorimager. As such, it was obsolete when I was an undergrad operating my lab&#039;s sequencer twenty years ago (radioactive sequencing had been struggling to compete with fluorescently labeled primers, and affordable four-color fluorescent dye terminators effectively ended the contest), and it became &lt;i&gt;really&lt;/i&gt; obsolete a decade or so ago, when the dominant technology shifted from gel electrophoresis to capillary sequencing. It&#039;s also important not to confuse &lt;i&gt;any&lt;/i&gt; of these approaches with so-called &quot;next-generation sequencing&quot;.

Basically: it&#039;s dirt cheap,but that&#039;s because it&#039;s a good twenty years out of date, and probably &lt;i&gt;no-one&lt;/i&gt; should buy it. I don&#039;t even know whether someone buying it could get ahold of the radioactive nucleotides it would require (they&#039;re actually very cheap, but they&#039;re heavily regulated), the film might be hard to get these days and isn&#039;t cheap, and the technology is decades out of date.I doubt it&#039;s been used for at least a decade; indeed, I suspect the reason it&#039;s being sold now is that it&#039;s finally been officially ruled as decontaminated, probably because there have been a dozen or more S35 half-lifes since it was last officially commissioned for use.</description>
		<content:encoded><![CDATA[<p>It&#8217;s a gel electrophoresis system for using radioactively labeled nucleotides, after which the gel can be dried and exposed to film or to phosphorimager. As such, it was obsolete when I was an undergrad operating my lab&#8217;s sequencer twenty years ago (radioactive sequencing had been struggling to compete with fluorescently labeled primers, and affordable four-color fluorescent dye terminators effectively ended the contest), and it became <i>really</i> obsolete a decade or so ago, when the dominant technology shifted from gel electrophoresis to capillary sequencing. It&#8217;s also important not to confuse <i>any</i> of these approaches with so-called &#8220;next-generation sequencing&#8221;.</p>
<p>Basically: it&#8217;s dirt cheap,but that&#8217;s because it&#8217;s a good twenty years out of date, and probably <i>no-one</i> should buy it. I don&#8217;t even know whether someone buying it could get ahold of the radioactive nucleotides it would require (they&#8217;re actually very cheap, but they&#8217;re heavily regulated), the film might be hard to get these days and isn&#8217;t cheap, and the technology is decades out of date.I doubt it&#8217;s been used for at least a decade; indeed, I suspect the reason it&#8217;s being sold now is that it&#8217;s finally been officially ruled as decontaminated, probably because there have been a dozen or more S35 half-lifes since it was last officially commissioned for use.</p>
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		<title>By: tofagerl</title>
		<link>http://boingboing.net/2013/01/17/government-surplus-dna-sequenc.html#comment-1630648</link>
		<dc:creator>tofagerl</dc:creator>
		<pubDate>Thu, 17 Jan 2013 23:01:00 +0000</pubDate>
		<guid isPermaLink="false">http://boingboing.net/?p=206335#comment-1630648</guid>
		<description>How about donating it to a public defenders office in a big city?</description>
		<content:encoded><![CDATA[<p>How about donating it to a public defenders office in a big city?</p>
]]></content:encoded>
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	<item>
		<title>By: daredelvis</title>
		<link>http://boingboing.net/2013/01/17/government-surplus-dna-sequenc.html#comment-1630644</link>
		<dc:creator>daredelvis</dc:creator>
		<pubDate>Thu, 17 Jan 2013 23:00:00 +0000</pubDate>
		<guid isPermaLink="false">http://boingboing.net/?p=206335#comment-1630644</guid>
		<description>Pretty old technology, and expensive to operate per base sequenced.  </description>
		<content:encoded><![CDATA[<p>Pretty old technology, and expensive to operate per base sequenced.  </p>
]]></content:encoded>
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		<title>By: WaferMouse</title>
		<link>http://boingboing.net/2013/01/17/government-surplus-dna-sequenc.html#comment-1630639</link>
		<dc:creator>WaferMouse</dc:creator>
		<pubDate>Thu, 17 Jan 2013 22:54:00 +0000</pubDate>
		<guid isPermaLink="false">http://boingboing.net/?p=206335#comment-1630639</guid>
		<description>Looking forward to the first happy mutant strain!</description>
		<content:encoded><![CDATA[<p>Looking forward to the first happy mutant strain!</p>
]]></content:encoded>
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		<title>By: Kevin Pierce</title>
		<link>http://boingboing.net/2013/01/17/government-surplus-dna-sequenc.html#comment-1630607</link>
		<dc:creator>Kevin Pierce</dc:creator>
		<pubDate>Thu, 17 Jan 2013 22:36:00 +0000</pubDate>
		<guid isPermaLink="false">http://boingboing.net/?p=206335#comment-1630607</guid>
		<description>eBay has them too:
http://compare.ebay.com/like/150432850681?var=lv&amp;ltyp=AllFixedPriceItemTypes&amp;var=sbar</description>
		<content:encoded><![CDATA[<p>eBay has them too:<br />
<a href="http://compare.ebay.com/like/150432850681?var=lv&#038;ltyp=AllFixedPriceItemTypes&#038;var=sbar" rel="nofollow">http://compare.ebay.com/like/150432850681?var=lv&#038;ltyp=AllFixedPriceItemTypes&#038;var=sbar</a></p>
]]></content:encoded>
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