At Neatorama, librarian John Farrier helpfully points out some places where fictional pony librarian Twilight Sparkle could stand to improve her professional practice. It is simultaneously a dedicated bit of pony fandom and an interesting overview of the many responsibilities of a real-world librarian.
Conducting a reference interview is the act of translating a patron's request into terms that are congruent with the library's resources. It may surprise non-librarians to learn this, but yes: reference interviewing is a skill. And it is one that Twilight should develop.
A good reference interview begins with the librarian conducting him/herself in a manner that is welcoming. Helping the patron is the first priority of a librarian working the reference desk. The patron is not a distraction or an annoyance. In the first reference interview in the series, Twilight interacts with her patron, Rainbow Dash. "Can I help you?" is a good beginning. But her tone and body language suggests that she would rather not.
… Twilight has some good reference interviewing sense. One pitfall that rookie librarians fall into is to give professional advice instead of information—especially medical and legal advice. In "Cutie Pox," Applejack and Applebloom visit the library and asking for medical advice. Twilight, aware that doing so could expose the library and herself to liability, deftly avoids doing so and refers Applejack and Applebloom to Zecora, a qualified medical professional.