Scientists combined multiple imaging technologies to deliver an unprecedented 3D view inside the body of crawling cancer cells, spinal cord circuit development, and immune cells traveling within a zebrafish (above). Nobel laureate Eric Betzig and his colleagues at the Howard Hughes Medical Institute integrate a technology called lattice light sheet microscopy with adaptive optics resulting in a very expensive, 10-foot-long microscope. From HHMI:
“It’s a bit of a Frankenstein’s monster right now,” says Betzig, who is moving to the University of California, Berkeley, in the fall. His team is working on a next-generation version that should fit on a small desk at a cost within the reach of individual labs. The first such instrument will go to Janelia’s Advanced Imaging Center, where scientists from around the world can apply to use it. Plans that scientists can use to create their own microscopes will also be made freely available. Ultimately, Betzig hopes that the adaptive optical version of the lattice microscope will be commercialized, as was the base lattice instrument before it. That could bring adaptive optics into the mainstream.
“If you really want to understand the cell in vivo, and image it with the quality possible in vitro, this is the price of admission,” he says.
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